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Category:3D graphics softwareThe 18S rRNA gene promoter sequence from Pneumocystis carinii and detection of gene expression.
The promoter sequence from the 18S ribosomal RNA (rRNA) gene of Pneumocystis carinii was determined and compared with the promoter region from Saccharomyces cerevisiae and E. coli. The P. carinii sequence contains conserved A + T-rich regions, which are absent in yeast and E. coli, and which are associated with the transcriptionally active form of the rDNA as well as the transcriptional control regions. The DNA sequence of the rRNA gene of P. carinii differs from those of other well-characterized fungi in the region from the promoter to the terminator. The 5′-end terminates transcription in this area of the gene, which is common to many eukaryotic rRNA genes. The transcription termination point is located downstream from the 5′-end terminus of the precursor 18S rRNA.NMR relaxation characterization of aqueous samples from aqueous mixtures of glycerol, D2O, and methanol-D2O: quantification of intermolecular interactions.
Recent evidence has suggested that D2O can destabilize the hydrogen-bond network in water. The present work provides a systematic NMR relaxation study of aqueous samples prepared from aqueous mixtures of glycerol, D2O, and methanol-D2O. The NMR relaxation properties of these samples reveal the presence of two modes of liquid-liquid immiscibility: (i) liquid-liquid immiscibility in the presence of methanol that is associated with a decrease in the self-diffusion coefficient D of the water, D2O, and glycerol (CD2O
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